LOCUS       pDONR/Zeo
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FEATURES             Location/Qualifiers
     misc_feature    complement(268..295)
                     /note="rrnB T2 transcription termination sequence (c)"
     misc_feature    complement(427..470)
                     /note="rrnB T1 transcription termination sequence (c)"
     primer_bind     537..552
                     /note="M13 Forward (-20) priming site"
     misc_recomb     570..668
                     /label=attL1
                     /note="attL1"
     misc_recomb     complement(2099..2195)
                     /label=attL2
                     /note="attL2 (c)"
     misc_signal     complement(2210..2229)
                     /note="T7 Promoter/priming site (c)"
     primer_bind     2237..2253
                     /note="M13 Reverse priming site"
     gene            2366..3175
                     /note="Kanamycin resistance gene"
     rep_origin      3296..3969
                     /note="pUC origin"
     vector          join(2107..3972,1..651)
                     /source="pDONR%99221"
                     /type="Donor Vector"
     misc_feature    669..691
                     /note="TEV site"
     source          692..2092
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /clone="MGC:163405 IMAGE:40146564"
                     /tissue_type="Pooled, cerebellum, kidney, placenta,
                     testis, lung, colon, liver, heart, thyroid, bladder,
                     uterus, PCR rescued clones"
                     /clone_lib="NIH_MGC_362"
                     /lab_host="DH10B"
                     /note="Vector: pCR4-TOPO with reversed insert; Clone
                     identification sequence tag: AAACGGTC"
     gene            692..2092
                     /gene="UGT2A3"
                     /db_xref="GeneID:79799"
                     /db_xref="HGNC:28528"
     CDS             692..2092
                     /dnas_title="UDP glucuronosyltransferase 2 family,
                     polypeptide A3"
                     /gene="UGT2A3"
                     /codon_start=1
                     /product="UDP glucuronosyltransferase 2 family,
                     polypeptide A3"
                     /protein_id="AAI30534.1"
                     /db_xref="GI:120660408"
                     /db_xref="GeneID:79799"
                     /db_xref="HGNC:28528"
                     /translation="MRSDKSALVFLLLQLFCVGCGFCGKVLVWPCDMSHWLNVKVILE
                     ELIVRGHEVTVLTHSKPSLIDYRKPSALKFEVVHMPQDRTEENEIFVDLALNVLPGLS
                     TWQSVIKLNDFFVEIRGTLKMMCESFIYNQTLMKKLQETNYDVMLIDPVIPCGDLMAE
                     LLAVPFVLTLRISVGGNMERSCGKLPAPLSYVPVPMTGLTDRMTFLERVKNSMLSVLF
                     HFWIQDYDYHFWEEFYSKALGRPTTLCETVGKAEIWLIRTYWDFEFPQPYQPNFEFVG
                     GLHCKPAKALPKEMENFVQSSGEDGIVVFSLGSLFQNVTEEKANIIASALAQIPQKVL
                     WRYKGKKPSTLGANTRLYDWIPQNDLLGHPKTKAFITHGGMNGIYEAIYHGVPMVGVP
                     IFGDQLDNIAHMKAKGAAVEINFKTMTSEDLLRALRTVITDSSYKENAMRLSRIHHDQ
                     PVKPLDRAVFWIEFVMRHKGAKHLRSAAHDLTWFQHYSIDVIGFLLTCVATAIFLFTK
                     CFLFSCQKFNKTRKIEKRE"
     misc_difference 1021..1021
                     /gene="UGT2A3"
                     /note="'A' in cDNA is 'G' in the human genome; no amino
                     acid change.  The chimpanzee genome agrees with the human
                     genomic sequence and not the cDNA."
     misc_feature    692..2092
                     /note="UGT2A3 coding region"
ORIGIN      
          1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
         61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
        121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
        181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
        241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
        301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
        361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
        421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
        481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
        541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
        601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
        661 AGCAGGCTct gaaaacttgt actttcaagg cgggaaagtc ctggtgtggc cctgtgacat
        721 gagccattgg cttaatgtca aggtcattct agaagagctc atagtgagag gccatgaggt
        781 aacagtattg actcactcaa agccttcgtt aattgactac aggaagcctt ctgcattgaa
        841 atttgaggtg gtccatatgc cacaggacag aacagaagaa aatgaaatat ttgttgacct
        901 agctctgaat gtcttgccag gcttatcaac ctggcaatca gttataaaat taaatgattt
        961 ttttgttgaa ataagaggaa ctttaaaaat gatgtgtgag agctttatct acaatcagac
       1021 acttatgaag aagctacagg aaaccaacta cgatgtaatg cttatagacc ctgtgattcc
       1081 ctgtggagac ctgatggctg agttgcttgc agtccctttt gtgctcacac ttagaatttc
       1141 tgtaggaggc aatatggagc gaagctgtgg gaaacttcca gctccacttt cctatgtacc
       1201 tgtgcctatg acaggactaa cagacagaat gacctttctg gaaagagtaa aaaattcaat
       1261 gctttcagtt ttgttccact tctggattca ggattacgac tatcattttt gggaagagtt
       1321 ttatagtaag gcattaggaa ggcccactac attatgtgag actgtgggaa aagctgagat
       1381 atggctaata cgaacatatt gggattttga atttcctcaa ccataccaac ctaactttga
       1441 gtttgttgga ggattgcact gtaaacctgc caaagctttg cctaaggaaa tggaaaattt
       1501 tgtccagagt tcaggggaag atggtattgt ggtgttttct ctggggtcac tgtttcaaaa
       1561 tgttacagaa gaaaaggcta atatcattgc ttcagccctt gcccagatcc cacagaaggt
       1621 gttatggagg tacaaaggaa aaaaaccatc cacattagga gccaatactc ggctgtatga
       1681 ttggataccc cagaatgatc ttcttggtca tcccaaaacc aaagctttta tcactcatgg
       1741 tggaatgaat gggatctatg aagctattta ccatggggtc cctatggtgg gagttcccat
       1801 atttggtgat cagcttgata acatagctca catgaaggcc aaaggagcag ctgtagaaat
       1861 aaacttcaaa actatgacaa gcgaagattt actgagggct ttgagaacag tcattaccga
       1921 ttcctcttat aaagagaatg ctatgagatt atcaagaatt caccatgatc aacctgtaaa
       1981 gcccctagat cgagcagtct tctggatcga gtttgtcatg cgccacaaag gagccaagca
       2041 cctgcgatca gctgcccatg acctcacctg gttccagcac tactctatag atTAGGACCC
       2101 AGCTTTCTTG TACAAAGTTG GCATTATAAG AAAGCATTGC TTATCAATTT GTTGCAACGA
       2161 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTTGCCAT CCAGCTGATA TCCCCTATAG
       2221 TGAGTCGTAT TACATGGTCA TAGCTGTTTC CTGGCAGCTC TGGCCCGTGT CTCAAAATCT
       2281 CTGATGTTAC ATTGCACAAG ATAAAATAAT ATCATCATGA ACAATAAAAC TGTCTGCTTA
       2341 CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG
       2401 ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGCG ATAATGTCGG
       2461 GCAATCAGGT GCGACAATCT ATCGCTTGTA TGGGAAGCCC GATGCGCCAG AGTTGTTTCT
       2521 GAAACATGGC AAAGGTAGCG TTGCCAATGA TGTTACAGAT GAGATGGTCA GACTAAACTG
       2581 GCTGACGGAA TTTATGCCTC TTCCGACCAT CAAGCATTTT ATCCGTACTC CTGATGATGC
       2641 ATGGTTACTC ACCACTGCGA TCCCCGGAAA AACAGCATTC CAGGTATTAG AAGAATATCC
       2701 TGATTCAGGT GAAAATATTG TTGATGCGCT GGCAGTGTTC CTGCGCCGGT TGCATTCGAT
       2761 TCCTGTTTGT AATTGTCCTT TTAACAGCGA TCGCGTATTT CGTCTCGCTC AGGCGCAATC
       2821 ACGAATGAAT AACGGTTTGG TTGATGCGAG TGATTTTGAT GACGAGCGTA ATGGCTGGCC
       2881 TGTTGAACAA GTCTGGAAAG AAATGCATAA ACTTTTGCCA TTCTCACCGG ATTCAGTCGT
       2941 CACTCATGGT GATTTCTCAC TTGATAACCT TATTTTTGAC GAGGGGAAAT TAATAGGTTG
       3001 TATTGATGTT GGACGAGTCG GAATCGCAGA CCGATACCAG GATCTTGCCA TCCTATGGAA
       3061 CTGCCTCGGT GAGTTTTCTC CTTCATTACA GAAACGGCTT TTTCAAAAAT ATGGTATTGA
       3121 TAATCCTGAT ATGAATAAAT TGCAGTTTCA TTTGATGCTC GATGAGTTTT TCTAATCAGA
       3181 ATTGGTTAAT TGGTTGTAAC ACTGGCAGAG CATTACGCTG ACTTGACGGG ACGGCGCAAG
       3241 CTCATGACCA AAATCCCTTA ACGTGAGTTA CGCGTCGTTC CACTGAGCGT CAGACCCCGT
       3301 AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA
       3361 AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT
       3421 TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTTC TTCTAGTGTA
       3481 GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT
       3541 AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC
       3601 AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA
       3661 GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA
       3721 AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG
       3781 AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT
       3841 CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG
       3901 CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT
       3961 TGCTCACATG TT
//
